ACTIVATING PROBIO STOCK SOLUTION CONCENTRATE
Purpose The primary reason to activate Probio stock solution concentrates is an economic one, extending the volume of product and increasing its cost-effectiveness. Adding a sugar cane source and culturing the microorganisms ensures that the microbes are active. Once the following procedure has been accomplished, the end result will be a full strength culture of SCD Probiotics.
In some applications, it may not be necessary to activate Probio stock solution. Consult your Probio representative if you have further questions.
• Airtight plastic container, or large tank
• 1 part Probio stock solution
• 1 part molasses,
• 20 parts chlorine-free water (for example, ¾ cup Probio stock solution, ¾ cup molasses in 4.5 liters of chlorine-free water.)
• pH paper for testing Molasses and pH papers are typically available through your supplier of Probio stock solution concentrates.
1. Dissolve molasses in warm water and add the Probio stock solution. Activating SCD Probiotics is a mostly anaerobic process, thus the presence of excessive oxygen is not desirable. Keep the activation as warm as possible. If you keep the SCD Probiotics between 30 °C – 35 °C it should activate in approximately 4 days. If the extension is kept between 20 °C – 30 °C then allow for 7-10 days. Depending on technique and extension conditions, it may take anywhere from 4 -14 days.
2. Check the pH to ascertain when the process is complete; the Probio stock solution is ready when the pH drops to 3.7 or below. Do not use SCD Probiotics that have not dropped below 4.0. If your pH continues to drop to 3.0 or even 2.0 this is normal and indicates high microbial activity.
3. The end product should smell slightly sweet and pickled.
4. Activated SCD Probiotics is best used within 7 days. It may last up to 1 (one) month but should be used within this timeframe.